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This workshop will present recent methods of mapping sequence reads generated by an RNA-seq experiment to a reference genome (human genome),assigning reads to genome features (exon, intron, etc), and using the expression levels of those features to identify differentially expressed genes between different conditions (cancer vs normal, treated vs untreated, etc). We will examine some of the methods, their principles and biases in every step. We will look at techniques to quantify our results, and some of the pitfalls to be aware of. At the end of this workshop, participants will have the ability to perform analysis of a realistic/own dataset, from data retrieval through differential gene expression, and have an appreciation of the available data sources and tools, be deeply aware of biases, and be able to use these insights to critically interpret the results.

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Objectives

1. To give participants a combination of lecture and hands-on bioinformatics analysis
2. To expose and introduce a basic concept of RNA-seq technology
3. To provide the fundamentals concepts of differential gene expression
4. To promote awareness of application of RNA-seq in research

Facilitators

Instructors

• Wan Fahmi Wan Mohamad Nazarie
• Ang Mia Yang

Committee

• Nurul Syakima Ab Mutalib
• Ryia Illani Mohd Yunos
• Norshahidah Mahamad Nazir
• Muhammad Nazirul ‘Azim Mohd Arpandi

BANK INFORMATION

AccountÌýÌýÌýÌýÌýÌýÌýÌýÌýÌýÌýÌýÌýÌý Bendahari Universiti Kebangsaan Malaysia

Account NoÌýÌýÌýÌýÌýÌýÌý 86-0008114-0

BankÌýÌýÌýÌýÌý CIMB Berhad

Bank AddressÌýÌýÌý CIMB Cawangan Taman Maluri, No. 279 & 279A, Jalan Perkasa 1,

Taman Maluri Off Jalan Cheras, 55100 WP Kuala Kumpur, Malaysia

CPB/GST ÿÈÕ´óÈüappÌýÌý 0017 8225 9712

Swift CodeÌýÌýÌýÌýÌýÌýÌýÌýÌý CIBB MYKL

Secretariat of ‘Introduction to Differential Gene Expression Analysis using RNA-seq’

Institut Perubatan Molekul ÿÈÕ´óÈüapp (UMBI)

Jalan Yaacob Latiff, Bandar Tun Razak

56000 Cheras, Kuala Lumpur, Malaysia

Fax : +603 – 9171 7185

For any inquiry, please directly contact

Dr. Wan Fahmi Wan Mohamad Nazarie Ìý(wanfahmi@ukm.edu.my)

Madam Ryia Illani (ryia.yunos@ppukm.ukm.edu.my)

The Bioinformatics Workshop will be held at ÿÈÕ´óÈüapp Medical Molecular Biology Institute (UMBI), Kuala Lumpur that is located just a 15-minute drive away from the heart of Kuala Lumpur city centre.

For more information about the venue, please visit the UMBI website at /umbi/.

Please take note that the accommodation is NOT included in the registration fee. Kindly refer to the Accommodation tab for further information.

WAZE:

GOOGLE MAP:

Hotel nearby UMBI:

Hotel Sri Permaisuri Cheras (3-star hotel)

Hotel EST Kuala Lumpur

International Youth Centre (IYC) Residence

Low Budget hotel are also available nearby the UMBI. Please refer to any hotel booking website for further information.

**Important Note: KINDLY BE NOTED THAT THE ORGANIZER WILL NOT BE LIABLE FOR ANY CLAIM OR RESPONSIBILITY IN REGARDS OF THE LISTED ACCOMMODATION ABOVE. FOR ACCOMMODATION BOOKING AND RESERVATION, PLEASE CLICK THE APPROPRIATE LINKS AND FOLLOW THE GUIDELINES IN THE RESPECTIVE WEBSITE.

Dr. Wan Fahmi Wan Mohamad Nazarie (wanfahmi@ukm.edu.my) or

Madam Ryia Illani (ryia.yunos@ppukm.ukm.edu.my)

Institut Perubatan Molekul ÿÈÕ´óÈüapp (UMBI)

Pusat Perubatan ÿÈÕ´óÈüapp (PPÿÈÕ´óÈüapp),

Jalan Yaacob Latiff, Bandar Tun Razak

56000 Cheras, Kuala Lumpur,

MALAYSIA

Fax: +603 – 9171 7185

WAZE:
GOOGLE MAP: