CRISPR Genome Editing Workshop 2022
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Facilitators

Dr Saiful Effendi Syafruddin
Research Fellow
Cancer Biology, Immunology & Functional Genomics
effendisy@ppukm.ukm.edu.my
+603-91459072
BSc. (Hons) Biotechnology (RIT, USA), MSc. Molecular Genetics (Uni of Pennsylvania, USA), PhD Medical Sciences (University of Cambridge)

Dr. Mohamad Aimanuddin Mohtar
Research Fellow
Cancer Biology and Functional Proteomics
m.aimanmohtar@ppukm.ukm.edu.my
+603-91459062
BSc. Biotechnology (H. Hons) (USA), MSc. Molecular Biology (Uni of Pennsylvania, USA), PhD Molecular & Clinical Medicine (Uni of Edinburgh, UK)
Sponsors

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Introduction
The UMBI 2022 CRISPR workshop is tailored to give an overview of the Nobel-prize winning genome editing technology combined with several hands-on laboratory practical. This workshop consists of several lecture sessions that will comprehensively cover the important background and principal aspects of CRISPR technology. These include CRISPR initial discovery as the mechanism of bacteria adaptive immune response, CRISPR repurposing for eukaryotic gene targeting, CRISPR wide range of applications in the field of molecular biology and life sciences, and CRISPR genome editing experimental workflow. The participants will also get hands-on experience as well as tips and tricks from the experts focusing on the key laboratory techniques within this technology.
When?
18-20th October 2022
8.30am – 4.30pmWhere?
ÿÈÕ´óÈüapp Medical Molecular Biology Institute (UMBI), Jalan Yaacob Latiff, 56000 Kuala Lumpur
Workshop fees
RM 950 (Non-Student – Scientist, researcher, specialist, academicians, industry partners etc)
RM 800 (Student)
Fees include workshop materials and meals (3 times a day)
Why should you join?
- Learn how to perform CRISPR genome editing experiment using the cost-effective conventional approach without completely relying on the commercial kits.
- Facilitated by researchers who have extensive CRISPR genome editing with successful results and high-impact publications.
- Opportunity to perform hands-on laboratory practical; choosing vector system, sgRNA designing and cloning, and delivering into cells. Important tips and troubleshooting strategies for a successful CRISPR experiment will also be shared.Ìý
- Free complete in-house CRISPR guidebook written by the facilitators
Who should attend?
We welcome all Researchers, Scientists, Postgraduate Students, Application Specialists, or anyone who is interested in learning this CRISPR technology to attend. Although it is not essential, having basic molecular biology knowledge would be an advantage as the course will cover few lectures and practical sessions that are in intermediate-to-advanced levels. Nonetheless, we will accommodate all participants who are keen to join the workshop and learn this CRISPR genome editing technology.
The workshop is limited to only 20 seats
Past workshop
We had a successful inaugural CRISPR genome editing workshop that was held on the 18-20th November 2019 (Link: /umbi/news/crispr-gene-editing-workshop-2019/). The workshop was attended by researchers and students from local and overseas institutions and has garnered several collaborations with participants who mainly come from the academia. In addition to the active collaboration with local and overseas researchers for CRISPR works, the facilitators have also been invited to give talks in several webinars and pre-conference workshops about this CRISPR genome editing technology.
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UMBI CRISPR Workshop (18-20 October 2022)
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Tuesday 18 October 2022 – Day 1
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TIME PROGRAMME 0800-0900 REGISTRATION AND BREAKFAST Ìý Ìý
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0900
Ìý ÌýLecture 1 : Introduction and principle of CRISPR/Cas9 technology
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·ÌýÌýÌýÌýÌýÌýÌýÌý Brief history
·ÌýÌýÌýÌýÌýÌýÌýÌý Genome editing tools
·ÌýÌýÌýÌýÌýÌýÌýÌý Principle of CRISPR-based genome editing
1015 SHORT BREAK Ìý Ìý
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1030
Ìý Lecture 2: Cas9 variants and applications of CRISPR/Cas9 technology
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·ÌýÌýÌýÌýÌýÌýÌýÌý Cas9 Nickase & Dead Cas9
·ÌýÌýÌýÌýÌýÌýÌýÌý Base Editing & Prime Editing
·ÌýÌýÌýÌýÌýÌýÌýÌý Research and Clinical Applications
·ÌýÌýÌýÌýÌýÌýÌýÌý Ethical Issues
1145 SHORT BREAK Ìý Ìý
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1200
Ìý Lecture 3: Starting your CRISPR experiment
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·ÌýÌýÌýÌýÌýÌýÌýÌý Developing Relevant Biological Questions
·ÌýÌýÌýÌýÌýÌýÌýÌý General Gene Knockout and Knock-In Experimental Designs
·ÌýÌýÌýÌýÌýÌýÌýÌý Choosing the Right CRISPR System
1300-1415 LUNCH BREAK 1415 ÌýPractical 1 – sgRNA design Ìý
oÌýÌýÌý Extracting genomic information (Ensembl Demo)
oÌýÌýÌý sgRNAs designing (CRISPick Demo)
oÌýÌýÌý sgRNAs target region visualization (Snapgene Viewer Demo)
oÌýÌýÌý Primer design to amplify targeted region
1630 TEA & END OF DAY 1 Wednesday 19 October 2022 – Day 2
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<tTIME PROGRAMME 0800-0900 BREAKFAST Ìý Ìý
Ìý
0900
Ìý ÌýLecture 4 : CRISPR-Cas9 experimental workflow
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·ÌýÌýÌýÌýÌýÌýÌýÌý Knockout/Knockin workflow
·ÌýÌýÌýÌýÌýÌýÌýÌý sgRNA cloning strategy
1030 SHORT BREAK Ìý Ìý
Ìý
1045
Ìý Practical 2
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oÌýÌýÌý Expression vector digestion
oÌýÌýÌý sgRNAs annealing
oÌýÌýÌý sgRNAs ligation
oÌýÌýÌý Bacteria transformation
1300-1415 LUNCH BREAK Ìý Ìý
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1415
Ìý Lecture 5: Cells delivery and selection of positive cells
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·ÌýÌýÌýÌýÌýÌýÌýÌý CRISPR components delivery into the cells
·ÌýÌýÌýÌýÌýÌýÌýÌý Determination of delivery efficiency
·ÌýÌýÌýÌýÌýÌýÌýÌý Antibiotic selection (pooled cells isolation)
·ÌýÌýÌýÌýÌýÌýÌýÌý Single cell sorting and dilution
1515-1530 SHORT BREAK 1530 Ìý Practical 3
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oÌýÌýÌý CRISPR plasmids transfection
oÌýÌýÌý T7E1 endonuclease assay (PCR product rehybridization)
1630 TEA & END OF DAY 2 Thursday 20 October 2022 – Day 3
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TIME PROGRAMME 0800-0900 BREAKFAST Ìý Ìý
0900
Ìý ÌýPractical 4
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oÌýÌýÌý Transfected cells visualization
oÌýÌýÌý T7E1 endonuclease assay (Digestion)
1015-1030 SHORT BREAK Ìý Ìý
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1030
Ìý ÌýSponsored Talk : PLT Scientific
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ÌýMultiplex Western Blot Imaging and More with Azure Gel Imaging System
1130-1145 SHORT BREAK Ìý Ìý
1145
Ìý Practical 5
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oÌýÌýÌý T7E1 endonuclease assay (Result discussion)
oÌýÌýÌý Azure Biosystem Demo (Western blot Imaging)
1300-1415 LUNCH BREAK 1415 Ìý Lecture 6: Characterization of edited cells and CRISPR-Cas9 case studies
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Networking and Discussion
1630 TEA & END OF WORKSHOP Link in PDF : UMBI CRISPR Workshop 2022 Tentative
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The CRISPR gene editing workshop will be held at ÿÈÕ´óÈüapp Medical Molecular Biology Institute (UMBI), Kuala Lumpur that is located just a 15-minute drive away from the heart of Kuala Lumpur city center.
For more information about the venue, please visit the UMBI website atÌý/umbi/.
Please take note that the accommodation isÌýNOTÌýprovided. Kindly refer to theÌýAccommodation tabÌýfor further information.
Getting here:
- Public transport:Ìý
The nearest MRT station is Taman Pertama/Taman Midah
The nearest LRT stations is Salak Selatan/Bandar Tun Razak
Then take a GRAB or taxi to UMBI.- By Car:
Participant can park at Zone L parking in the ÿÈÕ´óÈüapp Medical Center (see attached map)

WAZE:Ìý
GOOGLE MAP:Ìý
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Hotels nearby UMBI:
- Hotel Sri Permaisuri Cheras (3-star hotel)
- Hotel EST Kuala Lumpur
- International Youth Centre (IYC) Residence
- Hotel Maple Inn
- V Hotel Kuala Lumpur
- Crystal Garden Hotel
Low Budget hotels are also available nearby the UMBI. Please refer to any hotel booking website for further information.
**Important Note: KINDLY BE NOTED THAT THE ORGANIZER WILL NOT BE LIABLE FOR ANY CLAIM OR RESPONSIBILITY IN REGARDS OF THE LISTED ACCOMMODATION ABOVE. FOR ACCOMMODATION BOOKING AND RESERVATION, PLEASE CLICK THE APPROPRIATE LINKS AND FOLLOW THE GUIDELINES IN THE RESPECTIVE WEBSITE.
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Contact details:
Miss Siti Nur Hasanah: +603 9145 9087 / 9185Ìý (email: hasanahyusuf@ukm.edu.my)
ÿÈÕ´óÈüapp Medical Molecular Biology Institute (UMBI),
Jalan Yaacob Latiff, Bandar Tun Razak,
56000 Cheras, Kuala Lumpur, Malaysia